|
KMID : 0861020090240020087
|
|
Korea Journal of Herbology
2009 Volume.24 No. 2 p.87 ~ p.92
|
|
|
Protective Effects of Nelumbinis Semen Against Neurotoxicity fuduced by 6-Hydroxydopamine in Dopaminergic Cells
|
|
Kim Hyo-Geun
Oh Myung-Sook
|
|
|
Abstract
|
|
|
Objectives : This study was performed to evaluate the neuroprotective effect of water extracts from Nelumbinis semen (NSW) in dopaminergic cells.
Methods : We performed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, 2,2-azinobis3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) cation scavenging assay, and determination of total polyphenolic content to examine the antioxidant effects of NSW. We also evaluated the neuroprotective effects against 6-hydroxydopamine (6-OHDA)-induced toxicity using 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl-tetrazolium bromide assay (MIT) assay, trypan blue cytotoxicity assay, and nitric oxide assay in SH-SY5Y cells and tyrosine hydroxylase (TH) immunohistochemistry in primary rat dopaminergic neurons.
Results : NSW showed values of 184.80 and 92.90 g/mL in DPPH and in ABTS assays, respectively. NSW showed 1.05% of total polyphenol contents. NSW showed protective effect against 6-0HDA-induced neurotoxicity whereas no influence on cell viability at the concentration of 150 g/mL. NSW reduced NO generation while 6-OHDA produced it. Moreover, it protected rat dopaminergic neurons against 6-0HDA at a dose of 1 g/mL.
Conclusions : These results indicated that NSW has neuroprotective effect against 6-0HDA-induced neurotoxicity through antioxidant activity in dopaminergic cell culture.
|
|
|
KEYWORD
|
|
|
Nelumbinis Semen, dopaminergic neurons, 6-0HDA, neuroprotection
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|